The human B cell activating factor (BAFF) and APRIL(a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily. They are expressed by non-B cells, and are down-regulated by mitogenic stimulation.2 BAFF and APRIL bind to at least two receptors: TACI (transmembrane activator and CAML-interactor) and BCMA (B cell maturation antigen), both of which are restricted to B cells.3,4 Ligation of these receptors with recombinant BAFFdramatically increases IgM production by peripheral blood B cells 1. Recently a third receptor for BAFF (BAFF-R) was described.5 Cell surface BAFF can be cleaved to form a soluble trimeric molecule. 2BCMA-muIg binds to recombinant BAFF-muCD8 and can inhibit binding of this molecule to receptors on Raji cells.
Molecular Structure: A soluble molecule consisting of the extracellular (54aa) domain of human BCMA fused to the murine IgG2a Fc (232 aa). Predicted monomeric weight: 33kD.
Transfectant Cell Line: CHO
Functional Application: BCMA-muIg binds to recombinant BAFF-muCD8 and can inhibit this molecule's abiltiy to bind to receptors on Raji cells.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer
仕様
Size：25ug
Source Antigen：CHO cells
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
