The human B cell activating factor (BAFF) and APRIL (a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily. They are expressed by non-B cells and are down-regulated by mitogenic stimulation. BAFF and APRIL bind to at least two receptors: TACI (transmembrane activator and CAML-interactor) and BCMA (B cell maturation antigen), both of which are restricted to B cells. Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells. Recently a third receptor for BAFF (BAFF-R) was described. BAFF and BAFF-R knockout mice have a reduced number of mature B cells in the periphery. However, TACI and BCMA knockouts do not share this phenotype, suggesting that BAFF-R may be the primary receptor for BAFF in mice. Cell surface BAFF can be cleaved to form a soluble trimeric molecule. Levels of soluble BAFF correspond with levels of autoantibodies in Sjogren’s Syndrome. BCMA-muIg binds to recombinant BAFF-muCD8 and can inhibit binding of this molecule to receptors on Raji cells. BCMA IgG2a Fc, Fusion Protein is a soluble molecule consisting of the extracellular domain (54 amino acids) of human BCMA fused to murine IgG2a Fc (232 amino acids).
Applications:Suitable for use in ELISA. BCMA-muIg binds to recombinant BAFF-muCD8 and can inhibit this molecule's abiltiy to bind to receptors on Raji cells. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Binding Inhibition of Raji Cell Receptors:B0807-50B was tested for its ability to block the binding of B0003-67EX to receptors on Raji cells. 5x10e5 human Raji cells were washed and incubated for 45 min. on ice with 80ul of C2432-01H at 1ug/ml. Cells were washed 2 times and incubated with C2259-11D. Cells were then washed 3 times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.3 log10 fluorescent units when compared with a buffer control. This binding was 88% inhibited when B0003-67EX was pre-incubated with 50ug/ml B0807-50B.
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：CHO cells
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride ,150mM sodium chloride, 0.5mg/ml gentamicin sulfate.
