The human B cell activating factor (BAFF,TALL-1, Blys, THANK) and APRIL(a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily. They are expressed by non-B cells, and are down regulated by mitogenic stimulation(2). BAFF and APRIL bind to at least two receptors: TACI (transmembrane activator and CAML-interactor) and BCMA (B cell maturation antigen), both of which are restricted to B cells(3,4). Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells(1). Recently a third receptor for BAFF (BAFF-R) was described(5). BAFF and BAFFR knockout mice have a reduced numbers of mature B cells in the periphery, however TACI and BCMA knockouts do not share this phenotype, suggesting that BAFF-R may the primary receptor for BAFF in mice(8,9,10). Cell surface BAFF can be proteolytically cleaved to form a soluble trimeric molecule(2). Levels of soluble BAFF correspond with levels of autoantibodies in Sjogren's Syndrome(11). Recombinant BAFF(trn)-muCD8 binds to human cell surface TACI/BCMA/BAFFR
Functional Application: Human BAFF(trn)-muCD8 binds to human cell surface TACI/BCMA/BAFFR.
Human BAFF(trn )-muCD8/Biotin*l
Molecular Structure: A soluble molecule consisting of 151 (VQGPEET...c-term) aminoacids of the extracellular domain of human BAFF fused to the extracellular domain (167aa) of murine CD8 alpha, with a predicted monomeric weight of 38.5 kd. A portion of the extracellular region of BAFF was truncated to eliminate a potential protease cleavage site.
Transfectant Cell Line: CHO
Applications:Suitable for use in Flow Cytometry and ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer
仕様
Size：25ug
Source Antigen：CHO cells
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3.
