Synaptobrevin 2 (Vehicle-associated membrane, VAMP2), which is an 18kD integral membrane protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal. Synaptobrevin 2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein (residues 1-89) forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25.
Recombinant protein corresponding to aa1-89 of human VAMP 2, His- tag expressed in E. coli (NP_055047).
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:For long-term storage, store at -20°C. Aliquots are stable for 6 month after receipts at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥95% by SDS PAGE
Form：Supplied as a liquid in PBS, pH 7.4, 1mM EDTA
