Cyclophilin B is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Recombinant human cyclophilin B was expressed in E. coli (NP_000933) and purified by using conventional chromatography techniques.
Enzyme Activity:Specific activity is > 1,500 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：500ug
Source Antigen：Human, Recombinant E. coli
Grade：Highly Purified
Purity：≥95% by SDS-PAGE
Form：Supplied as a liquid in 20mM Tris, pH 8.0, 20mM sodium chloride, 0.5mM DTT, 10% glycerol.
