The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.
Recombinant human MIF, fused to His-tag at N-terminus, was cloned into an E. coli expression vector. It consists of 151 amino acids.
Molecular Weight: 16.6kD (151 amino acids)
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:100ug
Source Antigen:Human, Recombinant, E. coli
Grade:Highly Purified
Purity:≥ 95% by SDS-PAGE. Purified by conventional column chromatography.
Form:Supplied as a colorless, clear solution in PBS, pH 7.4.