Hsp104 is a molecular chaperone required for stress tolerance and for maintenance of [psi(+)] prions in the budding yeast Saccharomyces cerevisiae. Hsp104 can protect yeast cells against high temperature and high concentration of ethanol but mutation studies have shown this protein is not required for normal growth. Hsp104 was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques. It consists of 908 amino acids.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:100ug
Source Antigen:Saccharomyces cerevisiae, Recombinant, E. coli
Grade:Highly Purified
Purity:≥ 90% by SDS PAGE; Purified by using conventional chromatography techniques.
Form:Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 100mM sodium chloride, 2mM EDTA and 5% glycerol.