DNA topoisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription. This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antibodies are a specific marker in Scleroderma patients (specificity 98-100%) and are associated with the presence of diffuse skin involvement and pulmonary fibrosis. In human tissues the DNA topoisomerase I is initially synthesized as a protein with 100kD molecular weight. Most of this precursor is then proteolytically processed to a species with 70kD molecular weight from which the Scl-70 antigen has derived its name. Recombinant Human Scl-70 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 91,576 dalton. Recombinant Scl-70 is expressed with a 6x His tag and purified by proprietary chromatographic techniques.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Suitable for use in Western Blot. Other applications not tested.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ul
Source Antigen：Recombinant
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
