Pentraxins are a family of proteins emerging from genes that are conserved in their carboxy-terminal halves a pentraxin domain and are prototypical acute phase proteins with acquired novel amino-terminal domains. Pentraxins, include C reactive protein (CRP) and Serum Amyloid P component (SAP), which serve as indicators of inflammatory reactions as a result of the exposure of liver cells to cytokines, mainly interleukin-6 (IL-6). PTX3 or TSG14 or long pentraxin (human mature chain 364 aa, chromosome 3q25; ~43 kda ) is structurally related but different form classic pentraxins. PTX3 was clones as an IL-1 inducible gene in endothelial cells, and as TNF-inducible gene in fibroblasts called TSG14. The C-terminal half of PTX3 aligns with CRP and SAP, but the N-terminal half is unique. PTX3 is induced by LPS, IL-1 and TNF-alpha, but not IL-6. During an acute phase response induced by LPS, PTX3 is expressed in many organs (heart, skeletal muscle). PTX3 expression may be indicative of its role in cardiovascular and inflammatory pathology.
Source:Human PTX3/TSG14 protein (1-381aa), containing his-tag at the C-terminus, was expressed in mouse myeloma cell line NSO. Purified protein is ~41kD (size on SDS-PAGE, reducing ~45-50kD due to glycosylation).
Applications:Suitable for use in Western Blot as a positive control. Other applications not tested.
Recommended Dilution:Western Blot: Load 10ul/laneOptimal dilutions to be determined by the researcher.
Note: SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2X sample buffer per 10ul of the solution prior to heating and loading on gels. This preparation is not biologically active. It is not suitable for ELISA or other applications where native protein is required. This preparation is intended for qualitative purpose and not to serve as standard of known concentration. Do not freeze, thaw, or heat repeatedly.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ul
Grade：Purified
Purity：≥ 90%
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
