85-1457-89　PHD1, Human, Control Peptide (PH1, prolyl hydroxylase domain-containing protein 1, HIFPH1, HIF-prolyl hydroxylase 1, EGLN2, EGL nine homolog 2, EIT6, estrogen-induced tag 6, DKFZp434E026) 100ug　P3373-05A

特徴

• Oxygen is absolutely critical for the survival of mammalian cells. Hypoxia induced factor (HIF) is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. There are 3-types of alpha subunits (HIF1-3alpha) and one HIF-beta subunits. However, the three HIF-alpha subunits are regulated by oxygen in a similar fashion, i.e. by regulated stabilization of the alpha-subunits. Under normal conditions, HIF-alpha Prolines are hydroxylated at Pro-402 and Pro-564. This allows binding of von Hippel-Lindau (VHL), the substrate recognition component of the E3 ubiquinated ligase complex, subsequent ubiquitination and degradation of HIF-alpha by the proteasome. Under hypoxic conditions, hydroxylation of HIF-alpha is inhibited and this prevents HIF-alpha degradation. The enzymes responsible for HIF-hydroxylation are known as HIF-prolyl hydroxylases (PHD1-3 or HPH1, HPH2, and HPH3). The three PHDs have been identified to hydroxylate the motif, LXXLAP* with *P being the hydroxyproline. PHD1 (human 407-aa, chromosome 19; also known as Egl nine homolog 2, EGLN2 (Hypoxia-inducible factor prolyl hydroxylase 1) (HIF-prolyl hydroxylase 1) (HIF-PH1) (HPH-3) (Prolyl hydroxylase domain-containing protein 1) (PHD1) (Estrogen-induced tag 6) is a hydroxylates HIF-1 alpha at Pro-402 and Pro-564, and HIF-2 alpha. PHD1 is expressed abundantly in all tissues with highest expression in testis. It is expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.
• Source:22aa peptide of Human PHD1/EGLN2 (1)
• Location:~C-terminal
• Species Sequence Homology:Mouse, rat-95%. No significant sequence homology exist with other PHDs.
• Applications:Suitable for use in ELISA as a control peptide for P3373-05. Other applications not tested.
• Recommended Dilution:ELISA: Use 50-100ng of control peptide/wellOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.