HNE is a major product of endogenous lipid peroxidation. The w-6-family (linoleic and arachidonic acids) of polyunsaturated fatty acids may produce HNE as a result of free radical attack. HNE is a highly reactive compound and it can react with several functional groups on biological material, particularly sulfydryl groups to form thioester adduct and then hemiacetals. HNE may also react with histidine and lysine residues of proteins to form stable Michael addition-type of adducts. HNE-modified proteins may display an altered biological functions. Antibodies to HNE will help to visualize the HNE-adducts.
Applications:HNE-BSA conjugate can be used to coat ELISA plates (coat 1-10ug/ml) to test antibodies or used a known positive control for ELISA or Western (load 1-5ul/lane). BSA-HNE conjugate is ~65-70kD.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 10mM Tris-buffer, pH 7.5, 0.01% benzethonium chloride. Labeled with BSA.
