Glyceraldehyde-3-phosphate dehydrogenase,G3PDH/GAPD/G3PD (EC 1.2.1.12) catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). G3PDH is a cytosolic enzyme. It is thought to be a tetramer of identical chains ~36kD. G3PDH (mouse/rat/rabbit 333; human 335-aa,chromosome 12p13.31-p13.1). The enzyme is present in such widely separated forms as man, lobster, and E. coli. Its rate of evolutionary change is one of the slowest known.
Applications:Suitable for use in Western Blot. Not suitable for ELISA or other applications where native protein is required. Other applications not tested.
Recommended Dilutions:Western Blot: Heat once and load 10ul/lane and visualize with G8140-14B. This preparation is intended for qualitative purpose and not to serve as standard of known concentration. Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ul
Source Antigen：Rabbit muscle
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in SDS-PAGE sample buffer (reduced)
