GST’s are responsible for the metabolism of a broad range of xenobiotics and carcinogens. The enzyme catalyzes the reaction of glutathione with a wide variety of organic compounds to form thioethers, a reaction that is sometimes a first step in a detoxification process leading to mercapturic acid formation. GSTs play an important role defense against electrophilic chemicals generated by cellular oxidative reactions catalyzed by cytochrome P450 and other oxidases. GSTs are believed to defend against the highly reactive electrophiles that are formed as a result of cellular oxidative processes. Based on amino acid sequence similarities and antibody cross-reactivities, the mammalian cytosolic GSTs are divided into the following classes: alpha, mu, kappa, theta, pi, zeta, omega and sigma. GSTs exists as homodimers of ~25kD. GST A1-1 is mainly found in kidney, intestine, lung, and liver. GST M1-1 is restricted to liver and few other tissues. GST P1-1 has a widespread distribution in most tumor cells and tissues but it is absent in liver. The GST M1-1 is the type b allelic variant and corresponds to human GST Y
GST Pi/ GST P1/ GST3, a 210aa protein (chrm 11q13) is present in all tissues and cells, with the exception of red cells, in which only erythrocyte GST (GSTe) is observed. Its expression was increased in many tumors relative to matched normal tissue and abundantly expressed in human skin. The protein shares 86% sequence identity with GSTP. GST Pi show enzyme activity towards CDNB; acrolein; adenine propenal; BDPE; benzyl isothiocyante; EA; 4-vinylpyridine.
Source:Human GST-pi/GST P1-1 was expressed in E. coli and purified (> 95%). Under native conditions, GST-alpha is homodimeric protein of ~50kD and under reducing conditions ~25kD.
Applications:Suitable for use in Western Blot as a positive control. Other applications not tested.
Recommended Dilution:To visualize (load 10ul/lane) with antibodies (Cat# G8135-25A). Heat once prior to loading on gels (10ul/lane)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ul
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：Purified (> 95%)
Form：Supplied as a liquid in SDS-PAGE sample buffer (reduced).
