Glucagon is a member of a multigene family comprising of Secretin, Vasoactive Intestinal Peptide (VIP), Gastric Inhibitory Peptide (GIP) and others like Glicentin and Oxyntomodulin (OXM), which differs from glucagon by C-terminal octapeptide. The glucagon precursor contains at least 3 intervening sequences that divide the protein-coding portion into 4 regions corresponding to the signal peptide and part of the N-terminal peptide, the remainder of the N-terminal peptide and glucagon, glucagon-like peptide-1 (GLP1), and GLP2. The GLP 1 & 2 stimulates intestinal growth and up regulates villus height in the small intestine, concomitant with increased crupt cell proliferation and decreased enterocyte apoptosis. The two GLP's are mainly produced in the A cells of the Islets of Langerhans in response to a drop in blood sugar concentration
GLP1 Receptor, a 489aa peptide in mouse, 463aa in rat and human (chr 6p21), it serves as receptor for GLP1. The activity of this receptor is mediated by G protein which activate adenylyl cyclase. GLP1 Receptor is mainly expressed in pancreatic islets, stomach, and lung.
Source:17aa peptide of mouse GLP1 Receptor, conjugated to KLH
Localization:Mid-region
Applications:Suitable for use in ELISA. Other applications not tested..
Recommended Dilutions:ELISA: 50-100ng/well. Optimal dilutions to be determined by he researcher.
Species Sequence Homology:Human, Rat: 100%
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
