Cytochrome P450 (P450 or CYP) enzymes, a superfamilyof b-type heme-containing proteins found in organisms from alldomains of life, are major catalysts in the oxidative transformationof a diversity of endogenous and exogenous compounds. CYP enzymes play an important role in the metabolic activation of environmentalprocarcinogens or chemical carcinogenesis, these enzymes are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid.
CYP1B1 (Cytochrome P450 family 1, subfamily B, polypeptide 1) a 543aa enzyme in mouse, rat and human( chr:2p22) belongs to a multigene superfamily of monomeric mixed-function monooxygenases, responsible for the phase 1 metabolism of a wide range of structurally diverse substrates by inserting 1 atom of atmospheric oxygen into the substrate molecule, thereby creating a new functional group (e.g., -OH, -NH2, -COOH). This enzyme is involved in an NADH-Dependent electron transport pathway, It oxidizes a variety of structurally unrelated compounds and participates in the metabolism of an as-yet unknown biologically active molecule that is a participant in eye development. Cyp1B1 is expressed in many tissues, Defects in Cyp1B1 causes primary congenital Glaucoma, this recessive disease is characterized by large ocular globes resulting from increased intraocular pressure.
Antigen:14aa peptide of human CYP1B1, conjugated to KLH
Location~C-terminal
Species Sequence Homology:Human peptide sequence is 92% conserved in chimp protein. It is only 35% conserved in mouse/rat Cyp1B1.
Applications:Control peptide, because of its low mol. Wt (< 3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of affinity purified IgG or 1 ul antiserum) to confirm antibody specificity.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Human synthetic peptide
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
