Pentraxins family of proteins acquired the name from their ability to form pentameric (or decameric) structures formed by non-covalent interactions. C-reactive protein (CRP or PTX1; mature chain 206 aa; chromosome 1q21-23) nonglycosylated, ~24kD monomer and ~118kD pentamer) is a ubiquitous protein found in both vertebrates and invertebrates. Originally CRP was defined as a substance, observed in the plasma of patients with acute infections, that reacted with the C polysaccharide of the pneumococcus. It is one of the plasma proteins that are called acute phase reactants because of a pronounced rise in concentration after tissue injury or inflammation; in the case of CRP the rise may be 1000-fold or more. CRP is composed of 5 identical, 21,500-molecular weight subunits. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver; those cells produce CRP detectable on lymphocytes.
Source:A mixture of peptides from mouse CRP (1 peptide near the N-terminus and 1 peptide from C-terminus) were coupled to KLH. The two free peptides can be used for ELISA or antibody blocking.
Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot due to low MW <3kD. Other applications not tested.
Recommended Dilution:Elisa and Antibody Blocking: 5-10ug per 1ug C7907-15D (affinity purified antibody). Optimal dilutions to be determined by the researcher
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:100ug
Source Antigen:Mouse synthetic peptide
Grade:Purified
Purity:Purified
Form:Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.