85-1456-80　Core Binding Factor alpha 2, Human, Control Peptide (CBFA2) 100ug　C7900-73A

特徴

• The gene noggin encodes a member of one of at least four distinct gene families encoding secreted polypeptides that bind tomembers of the transforming growth factor-beta superfamily, suchas BMP, and inhibit the function of these signaling proteinsby preventing their interaction with receptors on the cell surface. Other antagonists with related functions includeChordin, Follistatin, Sclerostin and members of the DAN family. These BMP antagonists are assumed to be diffusibleand therefore potentially important in the establishment of BMP-activity gradients in vivo. Although structurally distinct, members of these gene familieshave in some cases similar ligand specificity and overlappingpatterns of expression, and in the case of Chordin and Nogginthese proteins apparently are capable of at least partial compensationfor each other. In addition to these multiple secreted BMP antagonists, there are other secreted proteins whose primaryfunction is to overcome this antagonism. Thus, thereis a highly complex system to regulate the bioavailability andconsequently the activities of BMPs in the extracellularspace
• BMPs are important regulators of key events in the processes of bone formation during embryogenesis, postnatal growth, remodeling and regeneration of the skeleton. The BMPs function by binding to a receptor complex that is found on all normal cells and is composed of type-I and –II receptors. The primary unit of bone formation is osteoblast, the bone forming cell. These osteoblast cells respond to physical loading by transducing signals that alter gene expression patterns. Cbfa (core binding factor), the osteoblast specific transcription factor plays an important role in osteoblast differentiation and function
• Cbfa 2/ Runx1/ AML 1 (11 spliced forms) a 450aa protein in rat, 451aa in mouse and 453aa in human (Chr. 21q22.3), It binds to the core site 5’-PYGPYGGT-3’ of a number of enhancers and promoters like murine leukemia virus, T-cell receptor enhancers, expressed in all tissues except brain and heart.
• Source:14aa peptide from human CBFA2. Location: ~C-terminus. Sequence is 100% conserved in only human CBFA2.
• Applications:ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.