85-1456-56　BIN 1b, Rat, Control peptide (Spag11b, Sperm associated antigen 11B, Antimicrobial-like protein Bin-1b, Spag11, Sperm-associated antigen 11) 100ug　B1200-01B

特徴

• Bin 1b: (68aa, Bin 1b gene) The rat ortholog of  human HE2  with 29.3% amino acid sequence identity, tends to be effective against invasive pathogenic microorganisms while simultaneously preserving spermatozoa from cytotoxic damage during epididymal transport, maturation, storage and fertility. Since, Bin 1b appears to have different roles in epididymis function and may not only offer an interesting lead for work in contraception but may also have therapeutic implications for sexually transmitted diseases.
• HE2 is a human epididymis specific sperm binding secretory protein expressed by SPAG11 gene (chromosome 8p23-p22), a 103aa protein adopts a typical antigenic pattern with a subacrosomal equatorial distribution on the sperm head, this localization of HE2 with Beta-defensins in the epithelial defense system of epididymal duct have developed mechanism to fight ascending infections, including local production of these antimicrobial peptides and proteins.
• The regionalized epididymal expression of the HE2 and its isoforms suggests that they exert their effects at different times in the controlled sequence of sperm modification, maturation, binding and a direct role in fertilization. HE2 represents 3 major isoforms, HE2-alpha, HE2-beta and HE2-gamma, all variants have a common 71 residue N terminus but various C termini, these variations at C termini is due to frame shifting deletions. Thus, identical sequences and deletion, start and stop points indicate that HE2 isoforms are derived from  alternative splicing of 8 or more exons of a single gene and working towards common microbial defense and sperm maturation to acquire fertilizing ability. The human HE2 protein and its isoforms are 28% conserved.
• A gene product of rat epididymal duct, BIN1B, has been suggested to represent the rat counterpart of human HE2 protein. As HE2, BIN1B also shows structural characteristics of beta-defensins.
• Source:13aa peptide from rat BIN 1B, conjugated to KLH
• Location:~C-terminal
• Species Sequence Homology:Control peptide is 100% conserved in mouse, 78% in chimp and human HE2 protein. No significant homology detected in other species.
• Applications:ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (See B1200-01C).
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.