85-1456-23　Dispase (Neutral Protease) 10mg　D3760

特徴

• I.U.B.: 3.4.24.28A metallo, neutral protease exhibits mild proteolytic action that makes the enzyme especially suitable for the preparation of primary and secondary (subcultivation) cell culture. This protease is also used as a secondary enzyme in cell isolation and tissue dissociation applications.
• Unit Definition:One Unit releases Folin positive amino acids equivalent to 1umole tyrosine per minute from casein at 37°C, pH 7.5.
• Specific Activity:≥4 units per mg dry weight
• Protein:As Reported
• Units/mg Protein:As Reported
• Caseinase:As Reported
• Absorbance A280:As Reported
• Endotoxin:As Reported
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.