85-1456-01　［取扱停止］Carbonic Anhydrase III, Recombinant, Human (CAIII, CA3, EC 4.2.1.1, Carbonatedehydratase III) 5ug　CAS No:9001-03-0　C1105-42

特徴

• Carbonic anhydrase is widespread in nature, being found in animals, plants, and certain bacteria. In animals it plays an important role in respiration by facilitating transport of CO2 and is involved in the transfer and accumulation of H+ and HCO3-. In chloroplasts of plant cells the role of carbonic anhydrase may be related to photosynthetic fixation of CO2.
• Mammalian carbonic anhydrases are of several forms differing in enzymatic properties, amino acid sequences and inhibitor binding. In erythrocytes there is usually present both a high activity and a low activity form. The pH-rate profiles and pH-binding curves indicate that the same group with pKa of approximately seven is involved in both forms. All the isoenzymes have a molecular weight of approximately 30,000 and contain one zinc atom per molecule. Although Deutsch et al. (1972) report six carbonic anhydrases in horse red blood cells, most interest has been focused on high and low activity types. Bovine erythrocytes contain two electrophoretically separable forms designated A and B in order of mobility. Both have a high order of activity similar to the human variant “C”. Lindskog et al. (1971) devote a section of their review to polymorphism and nomenclature (pg. 595).
• The outstanding characteristic of carbonic anhydrase is its very high turnover number. Zinc may be replaced by cobalt. Shinar and Navon (1974) found that the cobalt-substituted enzyme is dependent upon the state of oxidation of the metal. Appleton and Sarkar (1974) indicate the activity is related to the ionization of a group close to the zinc.
• Recombinant Human CAIII, Carbonic anhydrase III having a MW of 33.9kD is one of a group of ten zinc metalloenzymes that catalyse the reversible hydration of CO2 to form HCO3-. CAIII is a cytoplasmic isoenzyme, but is released into the circulation following injury. Recombinant human CAIII is expressed with an amino-terminal hexahistidine tag.
• Protein Family:Belongs to the alpha-carbonic anhydrase family.
• Protein Domains:IPR001148 Carbonic anhydrase, eukaryotic
• Protein links:Precursor- Protein structure and amino acid sequence:Dimers and aggregates:≤ 1% as determined by silver-stained SDS-PAGE gel analysis
• Endotoxin:≤ 0.1ng/ug (IEU/ug) of CAIII
• Protein quantitation was carried out by two independent methods:(1) 1. UV spectroscopy at 280nm(2) Analysis by RP-HPLC, using a standard solution of CAIII as a Reference Standard
• Storage & Stability:Human CAIII although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze-thaw cycles.