85-1455-85　Timentin (Ticarcillin Disodium/Potassium Clavulanate 15:1) 1g　CAS No:4697-14-7,61177-45-5　T5579

特徴

• Timentin is a suitable antibiotic for Agrobacterium counterselection and a mixture of two antibiotics: ticarcillin and clavulanic acid. Most wild Agrobacterium isolates have beta-lactamase activity which clavulanic acid exhibits activity towards.
• Many Agrobacterium isolates are highly susceptible to Timentin. Agrobacterium strains carrying the standard pBR beta-lactamase gene are also Timentin (clavulanic acid) susceptible. Timentin killing of Agrobacterium wild-type strains is three logs greater than with comparable doses of carbenicillin. In plant transformation experiments, 0.1mg/ml Timentin (15:1) is sufficient to counterselect Agrobacterium.
• The appropriate dose in tissue culture medium is 0.1 mg/ml (not 0.1 mg/liter) of 15:1 mixture or 0.2 mg/ml of 30:1 mixture. To improve efficacy (of any antimicrobial), solid media are best slightly dried before use. At these concentrations, Timentin exhibits no phytotoxicity toArabidopsis root cultures.
• Ticarcillin Disodium/Potassium Clavulanate =15:1
• Synonyms:Timentin; Ticarcillin; MM 14151; [2R-(2α,3Z,5α)]-3-(2-hydroxyethylidene)-7-oxo-4-oxa- 1-azabicyclo[3.2.0]heptane-2-carboxylate disodium/potassium salt
• Assay (HPLC):≥750ug/mg (ticarcillin, on dried basis)≥50ug/mg (clavulanic acid, on dried basis)
• Appearance:White to off-white to slight yellow powder
• Identification (HPLC):Positive
• Solubility:Colorless, clear, complete
• Moisture (Karl-Fischer):≤4.2%
• pH:5.5-7.5
• Related Substances:Largest impurity: ≤4%Second impurity: ≤2.5%
• Storage and Stability:Store at 4°C in hermetically sealed container, protected from light. Stable for 12 months after receipt at 4°C.