85-1455-64　［受注停止］Nourseothricin (NTC, Nurseotricin, Nourseotricin, clonNAT, Streptothricin Sulfate) 10mg　CAS No:96736-11-7　N5375-74

特徴

• A group of antibiotic substances produced by Streptomyces variants. They are composed of an amino sugar and different polypeptide chains and may have broad spectrum antimicrobial and some antiviral properties. Streptothricin antibiotic Nourseothricin (NTC) is used for a broad spectrum of viruses, bacteria and other unicellular or complex organisms. Preferred selection antibiotic for genetically modified Gram-positive and Gram-negative bacteria, yeast, filamentous fungi, protozoa, plants and many more. Selection of recombinant strains is based on inactivation of NTC by monoacetylation of b-amino group of the b-lysine by Nouresothricin N-acetyltransferase the product of the sat1 or nat1 genes.
• Synonyms:NTC; Nurseotricin; Nourseotricin; clonNAT; Streptothricin Sulfate
• Molecular Formula:C31H58N12O10• H2SO4 C19H34N8O8 •H2SO4
• Molecular Weight:Streptothricin F: 600.61Streptothricin D: 856.97
• Form:Supplied as a non-sterile powder. Reconstitute with ddH2O to 100mg/ml and sterile filter. Maximal solubility is 1g/ml.
• Purity:Components D&F (HPLC): ~90%; Components C&E (HPLC): ~10%
• Biological Activity:~110% standard Nr. 82
• Ash:~2%
• Elementary Analysis (Typical):C: 32.3%H: 5.7%N: 14.0%S: 6.7%
• Applications:Suitable for use in selection of recombinant Leishmania strains.
• Recommended Dilutions:Added to growth medium to a final concentration of 100ug/ml. Optimal dilutions to be determined by the researcher.
• Reconstitution:Reconstitute with ddH2O to 100mg/ml and sterile filter. Maximal solubility is 1g/ml.
• Selection:For selection of recombinant Leishmania strains Nourseothricin NTC is added to the growth medium to a final concentration of 100ug/ml.
• PubChem Chemical ID:Streptothricin D: 202550, Streptothricin F: 197034
• Table 1:* MIC: Minimal inhibitory concentration** IC50: Concentration inhibiting growth by 50%
• Group SpeciesMIC* Selection conc.ug/mlug/mlGram-negative bacteria Escherichia coli 2-12 50Francisella tularensis -5Pseudomonas aeruginosa 50 100Gram-positive bacteria Bacillus subtilis 5 50Enterococcus faecium 8-256 500Staphylococcus aureus -12 50Streptomycetes Streptomyces lividans 6 100Yeast Candida albicans 200 250-450Cryptococcus neoformans -100Hansenula polymorpha -100Pichia pastoris -100Saccharomyces cerevisiae 25 100Filamentous fungi Acremonium chrysogenum -25Sordaria macrospora -50Ustilago maydis -75Protozoa Leishmania tarentolae -100Phytomonas serpens -100Plasmodium falciparum 75*-Toxoplasma gondii -500Plants Arabidopsis thaliana 20 200Oryza sativa 20 200
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.