85-1455-63　［受注停止］Thialysine (S-Aminoethyl-L-cysteine hydrochloride) 98+% 5g　CAS No:4099-35-8　T5000

特徴

• The lysine analog, thialysine, has been implicated in the mechanism for the inhibitory effect of the on human acute leukemia Jurkat T cells. When Jurkat T cells were treated with thialysine (0.32-2.5mM), apoptotic cell death along with several biochemical events such as mitochondrial cytochrome c release, caspase-9 activation, caspase-3 activation, degradation of poly (ADP-ribose) polymerase, and DNA fragmentation was induced in a dose- and time-dependent manner. However, these thialysine-induced apoptotic events were significantly abrogated by an ectopic expression of Bcl-xL, which is known to block mitochondrial cytochrome c release. Decylubiquinone, a mitochondrial permeability transition pore inhibitor, also suppressed thialysine-induced apoptotic events. Comparison of the thialysine-induced alterations in the cell cycle distribution between Jurkat T cells transfected with Bcl-xL gene (J/Bcl-xL) and Jurkat T cells transfected with vector (J/Neo) revealed that the apoptotic cells were mainly derived from the cells accumulated in S and G2/M phases following thialysine treatment. The interruption of cell cycle progression in the presence of thialysine was accompanied by a significant decline in the protein level of cdk4, cdk6, cdc2, cyclin A, cyclin B1, and cyclin E. These results demonstrate that the cytotoxic activity of thialysine toward Jurkat T cells is attributable to not only apoptotic cell death mediated by a mitochondria-dependent death signaling pathway, but also interruption of cell cycle progression by a massive down-regulation in the level of cdks and cyclins.
• Synonyms:S-(2-Aminoethyl)-L-cysteine Monohydrochloride; NSC 186915;S-(2-Aminoethyl)-L-cysteine Hydrochloride;S-(2-Aminoethyl)-L-cysteine Monohydrochloride;S-(β-Aminoethyl)-L-cysteine Hydrochloride; Thialysine Hydrochloride
• Molecular Formula:C5H12N2O2S·HCl
• Molecular Weight:200.70
• Appearance:Light beige powder
• Purity (TLC):≥99%
• Solubility (H2O, 50mg/ml):Light tan, clear
• Melting Point:200-202°C
• Specific Rotation (a,24 D):[a]D24 = -6.5 ± 1º (C=1 in Water)
• Chloride:≤18% (AgNO3)
• Storage and Stability:Store at 4ºC. Stable for 12 months after receipt.