85-1455-05　MAPKAP Kinase 2, Control Peptide (Mitogen-activated Protein Kinase-activated Protein Kinase 2, MAP Kinase-activated Protein Kinase 2, MAPK-activated Protein Kinase 2, MAPKAPK2, MAPKAPK-2, MK2) 1mg　M2355-01A1

特徴

• Synthetic peptide corresponding to aa 310-325 of MAPKAP Kinase 2 (MTSALATMRVDYEQIK) (linear). Recognizes MAPKAP. Detects a single 44kD protein as indicated in crude extracts prepared from either unstimulated or TNFα stimulated human HeLa cell lysates. Corresponding antibody (M2355-01A) recognizes MAPKAP. Detects a single 44kD protein as indicated in crude extracts prepared from either unstimulated or TNFα stimulated human HeLa cell lysates. This sequence is conserved 100% in human, mouse, rabbit, rat, hamster, chicken, Xenopus and zebrafish.
• MAPKAP kinase-2 (or MK2) is an acronym for mitogen-activated protein kinase-activated proteinkinase-2. This enzyme is phosphorylated and activated by Erks and p38 MAPK in vitro. While multiple residues of MK2 are phosphorylated in vivo in response to stress, only four of theresidues Thr25, Thr222, Ser272 and Thr334 are phosphorylated by p38 in an in vitro kinase assay. MK2 is serine-threonine protein kinase that contains a proline rich sequence and twoputative SH3-binding sites. MK2 phosphorylates downstream components on a consensus Pro-X-Ser/Thr-Pro motif. The bestcharacterized MK2 substrate is hsp27. Although unknown, thephysiological function of MK2 may be to increase stress resistance. MK2 is an essential component in the inflammatory response that regulates biosynthesis of TNF-a at a posttranscriptional level and MK2 is rapidly phosphorylated and activated in response to cytokines, stress and chemotactic factors.
• Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.