Hypoxia-inducible factor-1 (HIF-1), identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1beta subunit and one of three subunits (HIF-1alpha, HIF-2alpha or HIF-3alpha). The activation of HIF-1alpha is closely associated with a variety of tumors and oncogenic pathways. HIF-1alpha consists of DNA binding domain (DBD domain), Dimerization domain and C-terminal regulatiory domains, including two transactivation domains (TAD), an oxygen-dependent degradation (ODD) domain, and inhibitory domains. HIF-1alpha (aa530-826) contains two TAD and inhibitory domain.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥ 95% by SDS-PAGE. Purified by conventional chromatography techniques.
Form：Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 1mM DTT
