The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
Number of Samples: 1 standard curve, 39 samples in duplicate or 2 standard curves and 30 samples in duplicate
Sensitivity: 60pg/ml
Range: 0.156-10ng/ml
Sample Volume: 100ul/well
Incubation Time: <4.5 hours
Wavelength Setting: 450nm
Materials Included:
Anti-Hsp70B′ Immunoassay Plate,
5X Hsp70B, Extraction Reagent,
Recombinant Hsp70B′ Standard,
Sample Diluent,
10X Wash Buffer,
Anti-Hsp70B′,
Anti-Hsp70B′ Diluent,
Anti-Rabbit IgG: HRP Conjugate,
Anti-Rabbit IgG: HRP Conjugate Diluent
TMB Substrate,
Acid Stop Solution,
Instruction Manual
Storage and stability:All reagents are stable as supplied at 4°C, except the Recombinant Hsp70B′ Standard, which should be stored at -20°C. For optimum storage, the Recombinant Hsp70B′ Standard should be aliquotted into smaller portions and stored at -20°C. Avoid repeated freeze/thaw cycles.
