Synthetic peptide CRMP1 antibody was raised against a 15 amino acid peptide from near the carboxy terminus of human CRMP1 (Genbank accession No. AAH07613).
Collapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins involved in neuronal differentiation and axonal guidance. CRMP2 was previously shown to mediate the repulsive effect of Sema3A on axons and to participate in axonal specification. The X-ray crystal structure of murine CRMP1 was determined at 2.1 resolution and demonstrates that CRMP1 is a bilobed 'lung-shaped' protein forming a tetrameric assembly.
Collapsin-response mediator proteins (CRMPs) are highly expressed in the developing brain where they play major roles in axonal outgrowth, neurite differentiation, and apoptosis. Their continued expression in areas of high synaptic remodeling such as the cerebellum, hippocampus, and the olfactory system suggests that these proteins may also be involved in adult brain plasticity. CRMP-1 was initially identified as a dihydropyrimidinase expressed exclusively in brain; later studies have shown that it is involved with neurotrophin (NT) 3-induced neurite formation and outgrowth. CRMP-1 localization switches from axonal to somatodendritic when neurons reach functional maturity, suggesting that it is involved in early neuronal differentiation as well as in later processes related to the survival or death of the newly generated neurons.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/ml; Blocks the antibody (Cat. #C7517-02) activity completely by incubating the peptide with equal volume of antibody for 30 min at 37°C
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.
