85-1453-67　Vascular Endothelial Growth Factor, Recombinant, Mouse (VEGF) 2ug　V2110-07C

特徴

• Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as Tumor Angiogenesis Factor or Vascular Permeability Factor. Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating Vasolidation via nitric oxide-dependent pathways. Alternative splicing of the mRNA for VEGF-A results in several isoforms of the protein being produced. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 percent. In contrast to other factors mitogenic for endothelial cells such as FGF-1 , FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF.
• Description:Recombinant Mouse VEGF produced in Sf9 insect cells is a double, glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 38814 Dalton. Recombinant Murine VEGF is purified by proprietary chromatographic techniques.
• Dimers and Aggregates:Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
• Biological Activity:Recombinant Mouse VEGF-Sf9 is fully biologically active when compared to standards. Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-2.0 ng/ml, corresponding to a specific activity of 1 x 106 Units/mg.
• Endotoxin:≤ 0.1ng/ug (IEU/ug) of VEGF-Sf9.
• Protein Content:Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm
• 2. Analysis by RP-HPLC, using a calibrated solution of VEGF as a Reference Standard.
• Solubility:It is recommended to reconstitute the lyophilized VEGF-Sf9 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Stability:Lyophilized Recombinant VEGF-Sf9 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Murine VEGF-Sf9 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.