85-1453-37　［取扱停止］tRNA Synthetase, Alanyl, Recombinant, Human 5ug　T8663-70

特徴

• Alanyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 108 kD protein is an autoantigen recognized by PL-12 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that epitope spreading occurs in the autoimmune PL-12 response such that even antibodies to an isolated alanyl-tRNA molecule can develop.
• Description:Recombinant Human PL-12 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 107,598 dalton. Recombinant PL-12 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
• Immunological Functions:1. Binds IgG-type human auto-antibodies
• 2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test).
• Applications:Western-Blot with monoclonal anti-hexa-His-tag antibody & Polymyositis sera.
• Coating Concentration:0.3-0.8ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
• Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze-thaw cycles.