85-1453-01 ［取扱停止］Stem Cell Factor, Recombinant, Human (SCF, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF) 2ug S7973-03
特徴
- Recombinant Human SCF produced in insect cells is a single, glycosylated polypeptide chain containing 165aa and having a molecular mass of 118.41kD containing a C-terminal His-tag (6x His).
- Biological Activity:The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is typically 2-5ng/ml.
- Protein Content:Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics)
- 2. Analysis by RP-HPLC, using a standard solution of SCF as a Reference Standard.
- Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O ,0.1% HSA or BSA. Aliquot and store at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
- Size：2ug
- Source Antigen：Recombinant, Sf9 insect cells
- Grade：Highly Purified
- Purity：≥ 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. Endotoxin: ≤ 0.1ng/ug (IEU/ug).
- Form：Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with sterile ddH2O ,0.1% HSA or BSA to ≥ 0.1mg/ml, which can then be further diluted with in other aqueous solutions.
- Molecular Weight Non Ab：18.41
- EU Commodity Code：30021019
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