85-1452-89　［取扱停止］SHP 1, Recombinant, Human, aa243-541 (SHPTP-1, SH2 containing protein tyrosine phosphatase 1, PTP1C, SH-PP1, HCP, SHP, PTPN6) 5ug　S1013-25B

特徴

• The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541). The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). Recombinant SHP-1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid(Met) is attached at N-terminus. Recombinant SHP-1 produced in E.Coli is a single,non-glycosylated polypeptide chain conjtaining 300 amino acids and having a molecular mass of 34.3kD.
• Sequence:MGFWEEFES LQKQEVKNLH QRLEGQRPEN KGKNRYKNIL PFDHSRVILQ GRDSNIPGSDYINANYIKNQ LLGPDENAKT YIASQGCLEA TVNDFWQMAW QENSRVIVMT TREVEKGRNKCVPYWPEVGM QRAYGPYSVT NCGEHDTTEY KLRTLQVSPL DNGDLIREIW HYQYLSWPDHGVPSEPGGVL SFLDQINQRQ ESLPHAGPII VHCSAGIGRT GTIIVIDMLM ENISTKGLDCDIDIQKTIQM VRAQRSGMVQ TEAQYKFIYV AIAQFIETTK KKLEVLQSQK GQESEYGNITY
• Activity:~5000 U/mg
• Unit Defenition:One unit will hydrolyze 1nanomole of p-nitrophenylphosphatate per minute at pH 7.5 at 37°C using 10mM of substrate.
• Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.