85-1452-85　［取扱停止］Secretin, alpha, Recombinant, Human (GST) 10ug　S0625-15

特徴

• Erythrocyte spectrin is the main component of the red cell membrane skeleton responsible for the shape and physical properties of red cells. Spectrin is composed of 2 subunits alpha and beta (280 and 246kD respectively). The human erithroid a-spectrin (2418 amino acids) consists of 22 repeating segments of about 106 amino acids in length. This protein was demonstrated to be a specific substrate for ubiquitina tion in vitro and in vivo. The main ubiquitination site on human a-spectrin was identified in the lysine 27 of the repeating segment 17 by sitedirected mutagenesis. Cell-free experiments using radiolabeled, biotinylated or native ubiquitin and cellular lysates from rabbit retyculocytes, human erythrocytes or k562 cells, showed that only monoubiquitination occurs at this site.
• Description:Recombinant fusion protein: Glutathione-S-Transferase (GST) and a fragment of human a-spectrin (from Lysine 1601 to Leucine 1818) containing repeat 17 and the ubiquitination target lysine 27. The total molecular weight of the fusion protein is 52.4kD.
• Application:Substrate for cell-free ubiquitination assays.
• Stability:rGST-alpha Spectrin although stable at 140C for 1 week, should be stored desiccated below -180C. Please prevent freeze-thaw cycles.