85-1452-67　Ribosomal Protein P0, Recombinant, Human (RPP0) 20ug　R2031-28R

特徴

• The ribosomal phosphoproteins(P protein antigens) are associated with the large ribosomal subunit and therefore are antigenic targets with a cytoplasmic localization. Three P proteins have been described: P0 with a molecular weight of 35kD, P1 (19 kD) and P2 (17 kD). Autoantibodies against ribosomal P proteins are present in 10 % of SLE patients. If anti-ribosomal P antibodies were to occur in the absence of other typical SLE associated autoantibodies, they may account for some patients with so-called ANA-negative lupus. It has been reported that lupus patients positive for anti-ribosomal P autoantibodies have a high frequency of CNS involvement, suggesting a marker use for these antibodies.
• Description:Recombinant Human Ribosomal Phosphoprotein P0 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 35,096 dalton. Recombinant Human Ribosomal Phosphoprotein P0 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
• Immunological Functions:1. Binds IgG-type human auto-antibodies
• 2. Standard ELISA test (checker-board analysis of positive/negative sera panels.
• Applications:Western-Blot with monoclonal anti-hexa-His-tag antibody & SLE sera (Systemic Lupus Erythematodes Disease).
• Coating Concentration:0.3-0.7ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
• Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze-thaw cycles.