85-1452-50　［取扱停止］Protein Kinase C, theta, Recombinant, Human (PKCt) 2ug　P9103-42H

特徴

• Recombinant Human PKC-teta produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-706 and having a molecular mass of 86kD. PKC-t is purified by proprietary chromatographic techniques. This protein is the full-length form of the protein with an amino terminal poly His-tag.
• Unit Definition:~1800 units/mg. One unit of PKCt activity transfers 1 nmole of phosphate to a PKC-t pseudosubstrate peptide per minute at 300C using. Recombinant human active PKC-teta also phosphorylates histone, MARCKS, moesin, RasGRP3, and PKC alpha and PKC epsilon pseudosubstrates. PKC-teta is a novel PKC and does not require calcium for activity. Activity may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application. PKC-teta is involved in T-cell activation and T-cell receptor signaling including T-cell mediated activation of NF-kB and AP-1.
• Storage:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze-thaw cycles.

仕様

• Size：2ug
• Source Antigen：Recombinant, SF9 insect cells
• Grade：Purified
• Purity：≥ 85% (SDS-PAGE)
• Form：Supplied as a liquid in 10mM Tris, pH 7.4, 0.1M sodium chloride, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF and 0.03% Brij-35.
• Molecular Weight Non Ab：86
• EU Commodity Code：30021019
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