85-1452-49　［取扱停止］Protein Kinase C, gamma, Recombinant, Human (PKCg) 10ug　P9103-25E3

特徴

• Recombinant Human PKC-gamma produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-676 and having a molecular mass of 80kD. PKC-g is purified by proprietary chromatographic techniques. This protein is the full-length form of the protein with an amino terminal poly His-tag.
• Unit Definition:~6000-8000 units/mg. One unit of PKC-gamma activity transfers 1 nmole of phosphate to a PKC substrate peptide (myelin basic protein 4-14) per minute at 30°C. Recombinant human active PKC-g also phosphorylates full length myelin basic protein, histone, MARKS, and GSK-3beta. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions.
• Storage:Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. Please avoid freeze-thaw cycles.

仕様

• Size：10ug
• Source Antigen：Recombinant, SF9 insect cells
• Grade：Highly Purified
• Purity：≥ 90% (SDS-PAGE)
• Form：Supplied as a liquid in 10mM Tris, pH 7.4, 0.1M sodium chloride, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF and 0.03% Brij-35.
• Molecular Weight Non Ab：80
• EU Commodity Code：30021019
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