85-1452-46　［取扱停止］Protein Kinase C, epsilon, Recombinant, Human (PKCe) 2ug　P9103-20G1

特徴

• Recombinant Human PKC-epsilon produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-737 and having a molecular mass of 87kD. PKCe is purified by proprietary chromatographic techniques. This protein is the full-length form of the protein with an amino terminal poly His-tag.
• Unit Definition:~3250 units/mg. One unit of PKC activity transfers 1 nmole of phosphate to a PKC substrate peptide per minute at 30°C. Recombinant human active PKC also phosphorylates full length myelin basic protein, MARKS, GAP-34, and raf-1 kinase. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

仕様

• Size：2ug
• Source Antigen：SF9 insect cells
• Grade：Highly Purified
• Purity：≥90% (SDS-PAGE)
• Form：Supplied as a liquid in 20mM Tris, pH 7.4, 0.1M sodium chloride, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF, 1mM benzamidine and 0.03% Brij-35, 20% glycerol.
• Molecular Weight Non Ab：87
• EU Commodity Code：30021019
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