85-1452-35　［取扱停止］PPARg, Recombinant, Human (Peroxisome Proliferator Activated Receptor, gamma, HUMPPARG, NR1C3, PPARG1, PPARG2) 2ug　P5505-01D

特徴

• The peroxisome proliferator activated receptors (PPARs) are ligand-activated transcription factors within the nuclear receptor superfamily which play important roles in adipogenesis, glucose homeostasis and inflammation. Three different isotypes can be distinguished: alpha, beta and gamma. PPAR-g is mainly expressed in adipose tissue and to lesser extent in colon, the immune system and the retina. Whereas PPARa operates in the catabolism of fatty acids in the liver, PPARg influences the storage of fatty acids in the adipose tissue and plays a role in adipocyte differentiation. PPARg binds to DNA as a heterodimer with retinoid X receptor (RXR), activating expression of target genes after binding ligand.
• Recombinant Human Peroxisome Proliferator Activated Receptor Gamma expressed in E. coli is fused to an amino terminal hexahistidine tag and has a molecular weight of 59.2kD.
• Biological Activity:Specific activity is 3-4 units/ml obtained by measuring the increase of NADPH in absorbance at 340nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 30ºC
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.