85-1452-10　［取扱停止］Phosphoinositide 3 Kinase, p85 alpha, p110 delta, Recombinant, Human (PI3K) 5ug　P4073-30B

特徴

• PI3K is highly expressed in cells of hematopoietic origin. It plays an important role in dendritic cell (DC) trafficking and in the activation of specific immunity. PI3K -/- mice showed a reduced ability to respond to chemokines, and had a selective defect in the number of skin Langerhans cells and in lymph node CD8alpha-DCs. In macrophages, the chemokine RANTES/CCL5 activates the small GTPase Rac1 and its downstream target PAK2. This response depends on Gi activation and largely on the subsequent triggering of PI3K. Distinct regions within the p101 regulatory subunit primary stucture are responsible for interaction with PI3K and Gbg. The PI3K binding site is confined to the N-terminus, whereas binding to Gbg is mediated by the C-terminus of p101. The PI3K catalytic subunit carries a N-terminal His6 affinity Tag, whereas the p101 adaptor subunit carries a N-terminal GST Tag. The molecular weight for p110g chain is 126.3kD (without Tag) and the p101 chain 101kD (without Tag).
• Biological Activity:~ 3 nmol/mg/min using phosphatidylinositol as the substrate.
• Storage:Recombinant Human PI3Kd although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze-thaw cycles.