85-1452-01　［取扱停止］Phosphoinositide 3 Kinase, Recombinant, Bovine, p110 alpha, p85 alpha (PI3K) 1ug　P4073-26C4

特徴

• PI3Ka plays a specific role in apoptosis in human colon cancer cells. Injection of neutralizing antibodies specific to PI3Ka into adenocarcinoma cells induced apoptosis, a response that was reverted by treating cells with caspase inhibitor. It was also shown that PI3Ka mediated phosphorylation of the p85a adapter reduces the lipid kinase activity of the heterodimer and this gives hints for PI3K-dependent signaling events not requiring production of 3'-phosphorylated phosphoinositides. PI3Ka is a key regulator of the initiation of keratinocyte differentiation. A decrease in PI3Ka activity results in a loss of keratinocyte adhesion to the extracellular membrane and the initiation of early phase differentiation. The PI3Ka catalytic and regulatory subunits are coexpressed in Sf9 insect cells. Recombinant Bovine Phosphoinositide 3-kinase alpha is a glycosylated protein having a molecular weight as follows: p85a chain 83.5kD, p110 chain 124.3kD.
• Biological Activity:~ 1 nmol/mg/min using phosphatidylinositol as the substrate.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.