85-1451-87　Osteocrin, Recombinant, Human 10ug　O8061-18

特徴

• Osteocrin is a recently identified secreted protein expression of which was only detected in bone, peaking just after birth and decreasing markedly with age. A 1280-bp mRNA encodes osteocrin producing a mature protein of 103 amino acids with a molecular mass of 11.4kD. In primary osteoblastic cell cultures osteocrin expression coincided with matrix formation then decreased in very mature cultures. Treatment of cultures with 1,25-dihydroxyvitamin D3 resulted in a rapid dosedependent down-regulation of osteocrin expression, suggesting direct regulation. Chronic treatment of primary cultures with osteocrin-conditioned media inhibited mineralization and reduced osteocalcin and alkaline phosphatase expression. These results suggest that osteocrin represents a novel, unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator.
• Description:The recombinant Human Osteocrin is produced with N-terminal fusion of HisTag. The Human Osteocrin His-Tagged Fusion Protein is 13.6kD containing 106 amino acid residues of the human Osteocrin and 16 additional amino acid residues – HisTag (bold). MRGSHHHHHH GMASHMVDVT TTEAFDSGVI DVQSTPTVRE EKSATDLTAK LLLLDELVSL ENDVIETKKK RSFSGFGSPL DRLSAGSVDH KGKQRKVVDH PKRRFGIPMD RIGRNRLSNS RG
• Specificity:The amino acid sequence of the recombinant human Osteocrin is 100% homologous to the amino acid sequence of the human Osteocrin without signal sequence.
• Applications:Western blotting, ELISA
• Reconstitution:Add 0.2 ml of dH2O and let the lyophilized pellet dissolve completely. Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.