85-1451-63　［取扱停止］MHC Class 1 Chain-related Gene B, Recombinant, Human (MICB) 10ug　M3886-76

特徴

• MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, T cells, and CD8+ T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response.
• Description:rHuMICB produced in E.Coli is a single, non-glycosylated polypeptide chain containing 326 amino acids and having a molecular mass of 37kD. The sequence contains the extracellular domain of the mature human MICB (amino acid residues Ala23 – Tyr312) MICB is purified by proprietary chromatographic techniques.
• Dimers and Aggregates:Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
• Biological Activity:Recombinant Human MICB is fully biologically active when compared to standards. Measured by its ability to bind MICB antibody in ELISA.
• Endotoxin:≤ 0.1ng/ug (IEU/ug) of Recombinant Human MICB.
• Protein Content:Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm
• 2. Analysis by RP-HPLC, using a standard solution of MICB as a Reference Standard.
• Solubility:It is recommended to reconstitute the lyophilized MICB in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Stability:Lyophilized Recombinant Human MICB although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution MICB should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.