85-1451-13　［取扱停止］JNK2 alpha1, Recombinant, Human (c-Jun N-terminal Kinase 2, JNK2, JNK2alpha, JNK2A, JNK-55, Mitogen-activated Protein Kinase 9, MAP Kinase 9, MAPK 9, MAPK9, PRKM9, p54a, p54aSAPK, Stress-activated Protein Kinase 1a, SAPK1a, SAPK, Stress-activated Protein Kinase JNK2) 10ug　J2599-89

特徴

• JNK2 is a soluble 45kD protein (amino acids 1-382) that is activated in response to cellular stress, radiation and growth factors. JNK2 binds to the amino terminal activation domains of c-jun or ATF2 regulating AP-1 transcriptional activity.
• Unit Definition:~125 units/mg. One unit of JNK2 1 activity is equal to 1 nanomole of phosphate transferred to recombinant ATF-2 fusion protein per minute at 30°C with a final ATP concentration of 100 µM. Recombinant active JNK2 1 is capable of autophosphorylation and also phosphorylates c-Jun at serine 63 and serine 73. Kinase activity may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application. JNK2 1 is shorter than JNK2 2, and contains a five nucleotide insertion in the C terminal region which causes a shift in the reading frame and subsequent premature termination.
• Amino Acid Sequence:MSDSKCDSQF YSVQVADSTF TVLKRYQQLK PIGSGAQGIV CAAFDTVLGI NVAVKKLSRP FQNQTHAKRAYRELVLLKCV NHKNIISLLN VFTPQKTLEE FQDVYLVMEL MDANLCQVIH MELDHERMSY LLYQMLCGIKHLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTACTNF MMTPYVVTRY YRAPEVILGM GYKENVDIWSVGCIMGELVK GCVIFQGTDH IDQWNKVIEQ LGTPSAEFMK KLQPTVRNYV ENRPKYPGIK FEELFPDWIFPSESERDKIK TSQARDLLSK MLVIDPDKRI SVDEALRHPY ITVWYDPAEA EAPPPQIYDA QLEEREHAIEEWKELIYKEV MDWEERSKNG VVKDQPSDAA VSSNATPSQS SSINDISSMS TEQTLASDTD SSLDASTGPLEGCRGGGS.
• Storage:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze-thaw cycles.