85-1451-06　［取扱停止］Intrinsic Factor, Recombinant, Human (IF) 2ug　I8445-02

特徴

• Recombinant Human Intrinsic Facor (IF, GIF, Cobalamin/Vitamin B-12 binding transport protein). Recombinant Human IF produced in baculovirus is a glycosylated, polypeptide chain containing having a molecular mass of 55,000 Dalton. rHuIF is fused to a hexa-histidine at the C-terminus and purified by proprietary chromatographic techniques for removal of bound Vitamin B-12.
• Biological Activity:Standard ELISA test (checker-board analysis of positive/negative sera panels), immunodot with anti-IF autoantibody positive patient sera. Can be used for solid ELISA and fluid phase assays, 0.25-0.65µg/ml. Suitable for biotinylation and iodination.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

仕様

• Size：2ug
• Source Antigen：Recombinant, Sf9 Insect Cells
• Grade：Highly Purified
• Purity：≥95% by RP-HPLC and SDS-PAGE.
• Form：Supplied as a liquid in 20mM HEPES, pH 8.0, 100mM sodium chloride, 20% glycerol.
• Molecular Weight Non Ab：55
• EU Commodity Code：30021019
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