85-1450-95　［取扱停止］Interleukin 12, Recombinant, Rat (IL-12, Cytotoxic Lymphocyte Maturation Factor 1, CTL Maturation Factor, TcMF, CLMF p35, Natural Killer Cell Stimulatory Factor 1, NFSK1, p35, T cell Stimulating Factor, TSF) 2ug　I8434-17A

特徴

• Interleukin 12 (IL-12) is a cytokine with important regulatory functions bridging innate and adaptive immunity. It has been proposed as an immune adjuvant for vaccination therapy of infectious diseases and malignancies. The inflammatory properties of IL-12 play an important role in the adjuvant. Interleukin 12 (IL-12) is a proinflammatory cytokine composed by two chains, p40 and p35.It plays a key role in the promotion of a Th1 immune response in the gastrointestinal mucosa. An enhanced expression of IL-12 mRNA in gastric mucosa has been reported in individuals infected by Helicobacter pylori.
• Recombinant Rat IL-12 is a glycosylated disulfide linked heterodimeric polypeptide containing 503aa and having a molecular weight of 70kD comprised of disulfide-bonded 35kD (p35) and 40kD (p40) subunits.
• Amino Acid Sequence:The sequence of the first five N-terminal amino acids was determined and found to be Arg-Val-Ile-Pro-Val at the p35 subunit and Met-Thr-Glu-Leu-Glu at the p40 subunit.
• Biological Activity:ED50 range=0.01-0.05ng/ml as determined by the dose dependent secretion of interferon-gamma from PHA and IL-2 activated splenocytes that have been costimulated with PMA.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.