85-1450-60　［取扱停止］Hirudin, Recombinant, Pichia pastoris 2ug　H4080-05

特徴

• Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech. Unlike heparin, Hirudin acts directly on thrombin rather than through other clotting factors.
• Recombinant Hirudin is derived from yeast and the polypeptide chain contains 65 amino acids, Mw is 6979.5 daltons. Recombinant Hirudin identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule and the absence of a sulfate group on the tyrosine at position 63. Recombinant Hirudin is purified by proprietary chromatographic techniques.
• Biological Activity:The biological activity was measured using chromogenic assay. 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific activity was found to be ≥14,000ATU/mg.
• Storage and Stability:Lyophilized powder is stable for 12 months at -20°C. Upon reconstitution with sterile ddH2O, aliquot and store at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) to the solution. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.