85-1450-42　［取扱停止］Heat Shock Protein 27, Recombinant, Human (HSP27) 10ug　H1830-51A1

特徴

• Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38). Recombinant HSP-27 produced in E.Coli is a single,non-glycosylated polypeptide chain containing 205 amino acids and having a molecular mass of 22.7kD. Recombinant Human Hsp27 was overexpressed in E. coli and purified by conventional chromatography.
• Immunological Activity:Immunoreactivity is confirmed by reaction with monoclonal mouse antibodies against HSP25/27.
• Sequence:MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK
• Stoage and Stability:May be stored at 4°C for short-term only. For long-term storage, add 0.1% HSA or BSA, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.