85-1450-01　［取扱停止］Formiminotransferase Cyclodeaminase, Recombinant, Human 20ug　F6002-20

特徴

• Formiminotransferase cyclodeaminase, a bifunctional enzyme of tetrahydrofolate synthesis, is the target antigen of anti-LC1 (liver cytosol antigen type 1) autoantibodies. Presence of LC1 autoantibodies is a marker for type 2 autoimmune hepatitis (for which anti-LKM 1/cytochrome P450 2D6 autoantibodies are a further marker). Serum LC1 autoantibody concentrations appear to fluctuate in parallel with aminotransferase levels, a particularly intriguing observation that suggests a possible role of LC1 autoreactivity in the pathogenic mechanism leading to hepatocyte injury.
• Description:Recombinant Human Formiminotransferase Cyclodeaminase 1 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 59,749 dalton. Recombinant LC-1 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
• Immunological functions:1. Binds IgG-type human auto-antibodies
• 2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot test).
• Applications:Western-Blot with monoclonal anti-hexa-His-tag antibody.and autoimmune hepatitis (AIH II) patient sera.
• Coating Concentration:0.35-0.7ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
• Storage Conditions:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°Cfor longer periods of time. Please avoid freeze-thaw cycles.