85-1449-55　［取扱停止］ENA78, Recombinant, Human (Epithelial Neutrophil Activating Peptide 78) 5ug　E2275-06

特徴

• Recombinant Human ENA-78 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8020 Dalton. Recombinant Human ENA-78 is purified by proprietary chromatographic techniques.
• Amino Acid Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be, Ala- Ala -Val-Leu-Arg. .
• Dimers and Aggregates:Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
• Biological Activity:Recombinant Human ENA-78 is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human peripheral blood neutrophils using a concentration of 5-10 ng/ml.
• Endotoxin:≤ 0.1ng/ug (IEU/ug) of Recombinant Human Epithelial neutrophil-activating protein 78
• Protein Content:Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm
• 2. Analysis by RP-HPLC, using a calibrated solution of ENA-78 as a Reference Standard.
• Solubility:It is recommended to reconstitute the lyophilized ENA-78 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Stability:Lyophilized ENA-78 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution ENA-78 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.