85-1448-87　［取扱停止］CENP-B, Recombinant, Human (Centromere Protein B) 20ug　C2615-12H

特徴

• Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENP-A to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies. CENP-B is the most important of the centromeric autoantigens: it has a molecular weight of approx. 66kD and plays an important role in the formation of the centromeric chromatin. CENP-B antibodies are present in the sera of up to 80% of patients with CREST syndrome.
• Recombinant Human CENP-B produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 80kD..
• Recombinant human CENP-B is expressed with a -6X His Tag and purified by proprietary chromatographic techniques.
• Immunological Functions:1. Binds IgG-type human auto-antibodies
• 2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
• Applications:Suitable for use in ELISA, Western Blot, for biotinylation and iodination.. Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.