85-1448-85　［取扱停止］CENP-A, Recombinant, Human (Centromere Protein A) 20ug　C2615-05B

特徴

• Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENP-A to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies. CENP-A is another important centromeric autoantigen in addition to CENP-B: it has a molecular weight of approx. 20 kD and is incorporated into centromeric chromatin due to its histone-like properties. CENP-A antibodies are an important marker for correct diagnosis of Scleroderma / CREST syndrome in CENP-B-autoantibody negative patients.
• Description:Recombinant Human CENP-A produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 17,015 dalton. Recombinant CENP-A is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
• Immunological Functions:1. Binds IgG-type human auto-antibodies
• 2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
• Applications:Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.
• Coating Concentration:0.15-0.4ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
• Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze-thaw cycles.